Our Methods article about our tips and tricks for optimized Single Molecule Localization Microscopy (SMLM, previously announced here when we preprinted it) has just been published in its final form. This is part of a special issue on super-resolution microscopy, thanks to Jan Tønnesen for the invitation to contribute. If you want to optimize sample preparation and imaging for STORM and DNA-PAINT of classic celular targets (microtubules, actin, clathrin-coated pits), check it out!