The neuronal cytoskeleton in physiology and disease
Christophe presented the work of the team at the Single Molecule Localization Microscopy Symposium 2017 in London. Great meeting with lots of SMLM technical and biological insight. More reports on Twitter with the #SMLMS2017 hashtag (photo: Andrei Aristov).
Fanny Boroni-Rueda, an Aix-Marseille University technician, has joined the lab today. She will bring her “magic touch” for neuronal culture and much more!
A new preprint is out today on bioRxiv. This collaboration with the Ricardo Henriques and Jason Mercer labs proposes a new metric to measure the quality of super-resolution images. Simply put, it compares the image to a reference diffraction-limited image, allowing to detect artefacts and missing features in the super-resolved image. We used it to determine when to stop a STORM acquisition when visualizing axonal actin rings, and to optimize the dye concentration in a DNA-PAINT experiment. The method, called NanoJ-SQUIRREL (Super-resolution Quantitative Image Rating and Reporting of Error Locations), is available as an easy to use, open-source plugin for the ImageJ/Fiji plugin software. Try it!
