MiFoBio 2018: the best microscopy workshop!

Christophe was lucky to spend a whole week at the “Microscopie Fonctionnelle en Biologie” aka MiFoBio workshop. Lots of fun attending dozens of cutting-edge workshops, trying super-resolution microscopes, discussing, DJing (!), and presenting the latest work from the lab.

More on Twitter: #MiFoBio2018

photo: @HenriquesLab
COS cell labeled for actin and microtubules, imaged using Gataca System’s LiveSR spinning disk microscope
COS cell labeled for actin and microtubules, imaged using Olympus’ SpinSR10 spinning disk microscope

Brève Science & Vie

Christophe was interviewed by journalist Anne Debroise of the French scientific magazine Science & Vie about a recent article from the Bock lab (Zheng et al., 2018) that provides the volume of an adult Drosophila fly brain by electron microscopy. You can read the short article here (in French, subscription required) or via the screenshot below.

New preprint: Get the best 3D localization microscopy with DAISY

We have a new preprint out! It’s a collaboration with the group of Sandrine Lévêque-Fort at ISMO (Orsay, France) based on the PhD work of Clément Cabriel. They previously used supercritical-angle fluorescence to measure the height of fluorophores at the proximity of the coverslip. This technique, called Direct Optical Nanoscopy with Axially Localized Detection (DONALD), could bring the resolution down to 15 nm for 3D localization microscopy. Now they have combined the SAF-based method with cylindrical lens astigmatism to obtain a robust and precise 3D localization of fluorophores over ~1.5 µm above the coverslip, retaining the key advantafges of DONALD: drift-free, tilt-insensitive and achromatic. The new technique, called Dual-view Astigmatic Imaging with SAF Yield (DAISY 😉), allowed to image in 3D the periodic scaffold of adducin and ß-spectrin along axons of cultured neurons, as you can see on the Figure below:
Cabriel_Preprint

Farewell to Nikki

We all said goodbye to Nikki who finished her Master’s internship at the end of July. Nikki is a great student, and she managed to get a PhD position in the lab of Ruud Toonen at the Center for Neurogenomics and Cognitive  Research in Amsterdam. Good luck for this new adventure!

Dominic will join us as an ICN PhD program laureate

We had the chance of having Dominic Bingham, currently a Master’s student in Alison Twelvetree’s lab in Sheffield, apply for a PhD in the lab through the “Integrative and Clinical Neuroscience” PhD program. Congratulation to Dominic who succeeded to be one of the three laureates this year! We can’t wait to have him the lab where he will study the organization of presynaptic actin. Thanks also to the Aix-Marseille University A*MIDEX which is funding the PhD program.

Christophe talks at the French Cell Adhesion Club meeting in Strasbourg

It was a pleasure to present at the 7th Cell Adhesion Club Meeting in Strasbourg. After three fascinating days hearing about cell adhesion and related cell biological processes by top scientists of the field, it was very interesting to see how key tools and concepts can also inform cellular neurobiology. See the #Stradh18 Twitter hashtag for more, thanks to @GoetzJacky for the invite!

photo @XavierTrepat
photo @christlet